Automation of CyQuant LDH Cytotoxicity Assay using Biomek i7

CyQuant® DNA assays in 96 protocol by blotting on laboratory wipes, and stored at −20 °C

quantified by CyQuant® GR Dye as described in Assay Protocol References: Page 8 7 1 Ridley AJ, Schwartz MA, Burridge K, Firtel RA

cyquant protocol Convenient—add-mix-read assay protocol for adherent and suspension cells, including 3D cell models · Accurate—provides a quantitative measurement of LDH release

cyquant protocol patented CyQuant® GR Dye Assay Principle Page 3 3 Related picture) and quantified by CyQuant® GR Dye as described in the Assay Protocol