Mevalonate kinase assay using DEAE-cellulose column

Therefore the pH of the buffer must be adjusted at the working temperature Table 2 Buffers for Anion-Exchange Chromatography pH range Buffer Mwt pKa @25 °C

chromatography on a Sephadex G- 200 column The elution volume for each protein is indicated in the table below Estimate the molecular

deae cellulose column chromatography Diethylaminoethyl cellulose is a positively charged reisin used in ion exchange chromatography a type of column chromatography

deae sephadex The pooled fractions from gel filtration column exhibited the highest specific activity were exposed to an anion exchange chromatography using DEAE